Protein Engineering Design and Selection

ORIGINAL ARTICLES:

Alan J. Bleasby and John C. Wootton

Construction of validated, non-redundant composite protein sequence databases
Protein Eng. 1990 3: 153-159; doi:10.1093/protein/3.3.153 [Abstract] [PDF] [Request Permissions]  

Ch. Betzel, A.V. Teplyakov, E.H. Harutyunyan, W. Saenger, and K.S. Wilson

Thermitase and proteinase K: a comparison of the refined three-dimensional structures of the native enzymes
Protein Eng. 1990 3: 161-172; doi:10.1093/protein/3.3.161 [Abstract] [PDF] [Request Permissions]  

Matthias Wilmanns, Edith Schlagenhauf, Bruno Fol, and Johan N Jansonius

Crystallization and structure solution at 4 Å resolution of the recombinant synthase domain of N(5'-phosphoribosyl)anthranilate isomerase:indole-3-glycerol-phosphate synthase from Escherichia coli complexed to a substrate analogue
Protein Eng. 1990 3: 173-180; doi:10.1093/protein/3.3.173 [Abstract] [PDF] [Request Permissions]  

Liisa Holm, Anu K. Koivula, Päivi M. Lehtovaara, Ari Hemminki, and Jonathan K.C. Knowles

Random mutagenesis used to probe the structure and function of Bacillus stearothermophilus alpha-amylase
Protein Eng. 1990 3: 181-191; doi:10.1093/protein/3.3.181 [Abstract] [PDF] [Request Permissions]  

Michael R. Sierks, Clark Ford, Peter J. Reilly, and Birte Svensson

Catalytic mechanism of fungal glucoamylase as defined by mutagenesis of Asp176, Glu179 and Glu180 in the enzyme from Aspergillus awamori
Protein Eng. 1990 3: 193-198; doi:10.1093/protein/3.3.193 [Abstract] [PDF] [Request Permissions]  

Nathalie Ballery, Philippe Minard, Michel Desmadril, Jean-Michel Betton, David Perahia, Liliane Mouawad, Len Hall, and Jeannine M. Yon

Introduction of internal cysteines as conformational probes in yeast phosphoglycerate kinase
Protein Eng. 1990 3: 199-204; doi:10.1093/protein/3.3.199 [Abstract] [PDF] [Request Permissions]  

In-Koo Rhee, K.S. Lee, and P.C. Huang

Metallothioniens with interdomain hinges expanded by insertion mutagenesis
Protein Eng. 1990 3: 205-213; doi:10.1093/protein/3.3.205 [Abstract] [PDF] [Request Permissions]  

Barbara Meckelein, Theo Nikiforov, Annette Clemen, and Heribert Appelhans

The location of inhibitory specificities in human mucus proteinase inhibitor (MPI): separate expression of the COOH-terminal domain yields an active inhibitor of three different proteinases
Protein Eng. 1990 3: 215-220; doi:10.1093/protein/3.3.215 [Abstract] [PDF] [Request Permissions]  

Wlodek Mandecki

A method for construction of long randomized open reading frames and polypeptides
Protein Eng. 1990 3: 221-226; doi:10.1093/protein/3.3.221 [Abstract] [PDF] [Request Permissions]  

PROTOCOL:

Frank W. Larimer, Richard J. Mural, and Thomas S. Soper

Versatile protein engineering vectors for mutagenesis, expression and hybrid enzyme formation
Protein Eng. 1990 3: 227-231; doi:10.1093/protein/3.3.227 [PDF] [Request Permissions]  

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