Protein Engineering Design and Selection

ORIGINAL ARTICLES:

Yasuo Takeuchi, Shuji Noguchi, Yoshinori Satow, Shuichi Kojima, Izumi Kumagai, Kin-ichiro Miura, Kazuo T. Nakamura, and Yukio Mitsui

Molecular recognition at the active site of subtilisin BPN': crystallographic studies using genetically engineered proteinaceous inhibitor SSI (Streptomyces subtilisin inhibitor)
Protein Eng. 1991 4: 501-508; doi:10.1093/protein/4.5.501 [Abstract] [PDF] [Request Permissions]  

Haruhiko Tamaoki, Yuji Kobayashi, Shigenori Nishimura, Tadayasu Ohkubo, Yoshimasa Kyogoku, Kiichiro Nakajima, Shin-ichiro Kumagaye, Terutoshi Kimura, and Shumpei Sakakibara

Solution conformation of endothelin determined by means of ¹H-NMR spectroscopy and distance geometry calculations
Protein Eng. 1991 4: 509-518; doi:10.1093/protein/4.5.509 [Abstract] [PDF] [Request Permissions]  

V. Saudek, A. Pastore, M. A. Castiglione Morelli, R. Frank, H. Gausepohl, and T. Gibson

The solution structure of a leucine-zipper motif peptide
Protein Eng. 1991 4: 519-529; doi:10.1093/protein/4.5.519 [Abstract] [PDF] [Request Permissions]  

William R. Pitt and Julia M. Goodfellow

Modelling of solvent positions around polar groups in proteins
Protein Eng. 1991 4: 531-537; doi:10.1093/protein/4.5.531 [Abstract] [PDF] [Request Permissions]  

José Saldanha and Daruka Mahadevan

Molecular model-building of amylin and -calcitonin gene-related polypeptide hormones using a combination of knowledge sources
Protein Eng. 1991 4: 539-544; doi:10.1093/protein/4.5.539 [Abstract] [PDF] [Request Permissions]  

Masahiro Shirakawa, Hiroshi Matsuo, and Yoshimasa Kyogoku

Intersubunit disulfide-bonded -Cro protein
Protein Eng. 1991 4: 545-552; doi:10.1093/protein/4.5.545 [Abstract] [PDF] [Request Permissions]  

S.E. Plyte and G.G. Kneale

The role of tyrosine residues in the DNA-binding site of the Pf1 gene 5 protein
Protein Eng. 1991 4: 553-560; doi:10.1093/protein/4.5.553 [Abstract] [PDF] [Request Permissions]  

C. Ferraz, F. Heitz, J. Sri Widada, E. Caron, A. Cave, and J.P. Liautard

Conformational stability of human skeletal tropomyosins modified by site-directed mutagenesis
Protein Eng. 1991 4: 561-568; doi:10.1093/protein/4.5.561 [Abstract] [PDF] [Request Permissions]  

Stephen C. Inglis, J.Guy Guillemette, Jeanette A. Johnson, and Michael Smith

Analysis of the invariant Phe82 residue of yeast iso-1-cytochrome c by site-directed mutagenesis using a phagemid yeast shuttle vector
Protein Eng. 1991 4: 569-574; doi:10.1093/protein/4.5.569 [Abstract] [PDF] [Request Permissions]  

S.E. Hilgen and G.J. Pielak

The function of the Saccharomyces cerevisiae iso-1-cytochrome c gene is independent of the codon at invariant residue Phe82 when the gene is present on a low-copy-number vector
Protein Eng. 1991 4: 575-578; doi:10.1093/protein/4.5.575 [Abstract] [PDF] [Request Permissions]  

Jack E. Baldwin, Stephen L. Martin, and John D. Sutherland

Site-specific forced misincorporation mutagenesis using modified T7 DNA polymerase
Protein Eng. 1991 4: 579-584; doi:10.1093/protein/4.5.579 [Abstract] [PDF] [Request Permissions]  

J.Guy Guillemette, Yuko Matsushima-Hibiya, Tom Atkinson, and Michael Smith

Expression in Escherichia coli of a synthetic gene coding for horse heart myoglobin
Protein Eng. 1991 4: 585-592; doi:10.1093/protein/4.5.585 [Abstract] [PDF] [Request Permissions]  

John D. Altman, Dennis Henner, Björn Nilsson, Sephen Anderson, and Irwin D. Kuntz

Intracellular expression of BPTI fusion proteins and single column cleavage/affinity purification by chymotrypsin
Protein Eng. 1991 4: 593-600; doi:10.1093/protein/4.5.593 [Abstract] [PDF] [Request Permissions]  

PROTOCOL:

Len Hall and David C. Emery

A rapid and efficient method for site-directed mutagenesis by PCR, using biotinylated universal primers and streptavidin-coated magnetic beads
Protein Eng. 1991 4: 601; doi:10.1093/protein/4.5.601 [PDF] [Request Permissions]  

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