Protein Engineering Design and Selection

REVIEW:

O.B. Ptitsyn

Kinetic and equilibrium intermediates in protein folding
Protein Eng. 1994 7: 593-596; doi:10.1093/protein/7.5.593 [PDF] [Request Permissions]  

ORIGINAL ARTICLES:

Vincent Mikol, Jörg Kallen, and Malcolm D. Walkinshaw

The X-ray structure of (MeBm₂t)¹-cyclosporin complexed with cyclophilin A provides an explanation for its anomalously high immunosuppressive activity
Protein Eng. 1994 7: 597-603; doi:10.1093/protein/7.5.597 [Abstract] [PDF] [Request Permissions]  

Joachim Jäger, Richard A. Pauptit, Ursula Sauder, and Johan N. Jansonius

Three-dimensional structure of a mutant E.coli aspartate aminotransferase with increased enzymic activity
Protein Eng. 1994 7: 605-612; doi:10.1093/protein/7.5.605 [Abstract] [PDF] [Request Permissions]  

Simon J. Hubbard, Karl-Heinz Gross, and Patrick Argos

Intramolecular cavities in globular proteins
Protein Eng. 1994 7: 613-626; doi:10.1093/protein/7.5.613 [Abstract] [PDF] [Request Permissions]  

Gudrun Luthardt and Cornelius Frommel

Local polarity analysis: a sensitive method that discriminates between native proteins and incorrectly folded models
Protein Eng. 1994 7: 627-631; doi:10.1093/protein/7.5.627 [Abstract] [PDF] [Request Permissions]  

A. Aszódi and W.R. Taylor

Secondary structure formation in model polypeptide chains
Protein Eng. 1994 7: 633-644; doi:10.1093/protein/7.5.633 [Abstract] [PDF] [Request Permissions]  

Dan Donnelly, John P. Overington, and Tom L. Blundell

The prediction and orientation of -helices from sequence alignments: the combined use of environment-dependent substitution tables, Fourier transform methods and helix capping rules
Protein Eng. 1994 7: 645-653; doi:10.1093/protein/7.5.645 [Abstract] [PDF] [Request Permissions]  

Barbara Walker, John Kasianowicz, Musti Krishnasastry, and Hagan Bayley

A pore-forming protein with a metal-actuated switch
Protein Eng. 1994 7: 655-662; doi:10.1093/protein/7.5.655 [Abstract] [PDF] [Request Permissions]  

M.G. Grütter, J.van Oostrum, J.P. Priestle, E. Edelmann, U. Joss, U. Feige, K. Vosbeck, and A. Schmitz

A mutational analysis of receptor binding sites of interleukin-1ß:differences in binding of human interleukin-1ß muteins to human and mouse receptors
Protein Eng. 1994 7: 663-671; doi:10.1093/protein/7.5.663 [Abstract] [PDF] [Request Permissions]  

Niki J. Dembowski and Evan R. Kantrowitz

The use of alanine scanning mutagenesis to determine the role of the N-terminus of the regulatory chain in the heterotropic mechanism of Escherichia coli aspartate transcarbamoylase
Protein Eng. 1994 7: 673-679; doi:10.1093/protein/7.5.673 [Abstract] [PDF] [Request Permissions]  

Isabelle Quinkal, Valérie Davasse, Jacques Gaillard, and Jean-Marc Moulis

On the role of conserved proline residues in the structure and function of Clostridium pasteurianum 2[4Fe–4S] ferredoxin
Protein Eng. 1994 7: 681-687; doi:10.1093/protein/7.5.681 [Abstract] [PDF] [Request Permissions]  

G. Travé, D. Cregut, C. Lionne, J.-F. Quignard, L. Chiche, J. Widada, and J.-P. Liautard

Site-directed mutagenesis of a calcium binding site modifies specifically the different biochemical properties of annexin I
Protein Eng. 1994 7: 689-696; doi:10.1093/protein/7.5.689 [Abstract] [PDF] [Request Permissions]  

Yoram Reiter, Ulrich Brinkmann, Keith O. Webber, Sun-Hee Jung, Byungkook Lee, and Ira Pastan

Engineering interchain disulfide bonds into conserved framework regions of Fv fragments: improved biochemical characteristics of recombinant immunotoxins containing disulfide-stabilized Fv
Protein Eng. 1994 7: 697-704; doi:10.1093/protein/7.5.697 [Abstract] [PDF] [Request Permissions]  

William R. Trumble, May Huang, James W. West, Jennifer L. Reasoner, Jun-Ling Huang, Chang-Lin Wang, Richard J. Douthart, and Dale C. Birdsell

Gene synthesis and functional expression of a protein exhibiting monodomain IgG Fc binding
Protein Eng. 1994 7: 705-713; doi:10.1093/protein/7.5.705 [Abstract] [PDF] [Request Permissions]  

Chang-Lin Wang, May Huang, Carla A.R. Wesson, Dale C. Birdsell, and William R. Trumble

A single Fc binding domain–alkaline phosphatase gene fusion expresses a protein with both IgG binding ability and alkaline phosphatase enzymatic activity
Protein Eng. 1994 7: 715-722; doi:10.1093/protein/7.5.715 [Abstract] [PDF] [Request Permissions]  

W. Sangrar, S.M. Marcovina, and M.L. Koschinsky

Expression and characterization of apolipoprotein(a) kringle IV types 1, 2 and 10 in mammalian cells
Protein Eng. 1994 7: 723-731; doi:10.1093/protein/7.5.723 [Abstract] [PDF] [Request Permissions]  

PROTOCOL:

Ken Nishikawa, Sonoko Ishino, Hiromi Takenaka, Naoko Norioka, Teruhisa Hirai, and Toru Yao

Constructing a protein mutant database
Protein Eng. 1994 7: 733; doi:10.1093/protein/7.5.733 [PDF] [Request Permissions]  

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